Metadata

Barbatula undescribed species

[Behrmann-Godel, Nolte, Kreiselmaier, Berka and Freyhof 2017]

Barbatula undescribed species
Photograph by J. Behrmann-Godel
ORDERSUB-ORDERFAMILY
CypriniformesCobitoidei Nemacheilidae

Synonyms

None.

None. It is clear from the genetic information gained that this species is genetically isolated from close populations of Barbatula barbatula (the Stone Loach).

Country

Germany

Types

This species is not yet described so there are no types. The specimens used in the first account are deposited in Zoologisches Forschungsmuseum A. Koenig (ZFMK), Bonn (no catalogue numbers).

Distribution

Known only from the type locality: The Aach Spring, near Tuttlingen, southern Germany, (47o50’48.48”N, 8o51’28.91”E). This cave carries water from sinks in the Danube River, and elsewhere in the region.

Most subterranean fishes are tropical and subtropical and in the northern hemisphere the great majority are concentrated between 16oN and 25oN with only a handful north of this (Proudlove 2006 Table 3, Figure 1). Among the northerly outliers are populations of Amblyopsis spelaea, Troglichthys rosae and Typhlichthys lineages (Amblyopsidae) in North America, Cottus specus and an undescribed species in the Cottus bairdi-cognatus complex (Cottidae), also in North America, Troglocobitis starostini (Nemacheilidae) in Turkmenistan and Cobitis damlae (Cobitidae) in Turkey. At 47oN the German Barbatula is fully 6o further north than a population of Cottus bairdi-congatus complex in xxx. This great stride north, and into significantly glaciated terrain, opens the door to a host of temperate subterranean species so far undiscovered. For this reason alone this is probably the most significant discovery since Amblyopsis spelaea in 1840.

Habitat

The Aach Spring is the resurgence for the major Danube-Aach hydrological system (Hötzl 1996). It is a major phreatic conduit with no vadose passage and no significant dry passages. It has been explored for a distance of 600m, to a maximum depth of 40m, to where the passage is blocked with breakdown. The nearest sinks are c. 12km distant and the catchment is of c. 250km2. There is clearly a great deal of habitat space within the Danube-Aach System. The nature of the known habitat can be seen in this film. The large catchment, and especially the major inputs from the Danube sinks, will ensure a good food supply for the fishes even though the known population is a the extreme distal end of the system.

Systematics

Genetic analysis using mitochondrial COI markers (Fig. 1D) shows that the cave fishes share markers with surface living Barbatula barbatula from the region and this suggests that the cave fishes are recently evolved. However, population genetic analysis using microsatellite markers (Fig. 1E) shows that the cave fishes are genetically isolated from both upstream and downstream populations of B. barbatula. They also have reduced genetic variability, a feature they share with other cave fish populations (Sbdordoni, Allegrucci and Cesaroni 2000, 2005). Genetically isolated populations are species, by both the Evolutionary Species Concept and the Phylogenetic Species Concept, and this animal should be described as a new species. Recent glacial activity in the area suggests that the cave fishes have evolved post-glacially in perhaps as little as 20000 years though this requires further evidence for certainty.

Conservation Status

NE

This population is probably completely isolated and may be the only one. It may merit VU D2, at least, if there are any threats.

Museum Holdings

As above only.

Key References

Smyly, WJP Journal Article 1955 On the biology of the Stone Loach Nemacheilus barbatula (L.)
Bacesu-Mester, L Journal Article 1967 Contributions to the study of the genus Noemacheilus (Pisces, Cobitidae)
Perrin, JF Journal Article 1980 Structure et functionement des ecosystemes du Haut-Rhone francaise. 14. etude des preferences alimentaire de la loche franche (Noemacheilus barbatulus L.) par une methode des points modifee
Bless, R Journal Article 1985 Zur regeneration von Blachen der Agarlandschaft - Eine ichthyologische Fallstudie
Maitland, PS and Campbell, RN Book 1992 Freshwater fishes of the British Isles
Hotzl, H Journal Article 1996 Origin of the Danube-Aach system
Bromidge, N Book Section 2004 Stone loach Barbatula barbatula
Kottelat, M and Freyhof, J Book 2007 Handbook of European freshwater fishes
Behrmann-Godel, J., Nolte, A.W., Kreiselmaier, J., Berka, R. and Freyhof, J. Journal Article 2017 The first European cave fish
Coghlan, A. Web Page 2017 First ever cave fish discovered in Europe evolved super-fast
Behrmann-Godel, J. and Nolte, A.W. Journal Article 2018 Evolutiv junge europäische Höhlenfische

Internet Resourses

Webiste of the cave explorers

Account of the discovery

Andy Coghlan New Scientist

Practical Fish Keeping

Film of the dive and the fishes

Figure 1. From Behrmann-Godel, Nolte, Kreiselmaier, Berka and Freyhof 2017 (used with permission)
Cave loaches adapted to life in constant darkness are of recent origin but genetically divergent from surface populations residing in the same drainage. (A) Two cave loaches in their natural habitat. (B) Adult male loach with typical adaptations to living in caves: reduced eyes, enlarged barbels and pale body colouration. (C) Typical epigean loach from the surface population in the Danube. (D) Neighbour-joining tree of Cytochrome oxidase subunit1 (COI) sequences for European stone loaches. European loaches form three clusters comprising sequences from Rhine, Danube and Elbe drainages. Loaches from the Danube–Aach cave system (AC, highlighted in orange) share haplotypes with individuals from the southern lineage sampled in the upper Rhine and Danube drainage, individuals from GenBank identified by their accession numbers. Numbers at major nodes indicate bootstrap values (>50%, 1000 replicates). (E) Graphical representation of an analysis of genetic population structure. Inferred genomic ancestry in three genetic clusters (y-axis) is depicted by red, green and blue colours for all individuals (x-axis). With prior information on the sample location of individuals, three clearly distinct genetic clusters separate cave loaches from both surface populations upstream (Danube) and downstream (Radolfzeller Aach) in the same drainage. Without prior information on the sample location of individuals, cave fish are grouped with the upstream but clearly separated from the downstream loach population.
Figure 2. From Behrmann-Godel, Nolte, Kreiselmaier, Berka and Freyhof 2017 (used with permission)
(A) Map showing the hydrology of the Danube-Aach system including the Aach Spring catchment area (dashed blue line) and several Danube sinkholes (blue dots) discharging to Aach Spring (Simplified from Fig.3 in Hotzl (1996) (B) Cross section through the diving area in the main canal of the Danube-Aach underground system between Aach Spring and the collapse (Simplified from Schetter 2002 www.aachhoehle.de). Red circles indicate the sampling locations of 5 cave loaches. Arrows indicate direction of water flow from Danube to Aach Spring.