Journal Article

Karyotype and genome size of an endangered cavefish (Triplophysa rosa Chen & Yang, 2005)

Niu, Y., Zhao, Q., Zhao, H., Ludwig, A. and Peng, Z.

Record Number:
3975
Year:
2016
Journal:
Journal of Applied Ichthyology
Pages:
124-126
Volume:
33
Abstract:
The karyotype and genome size are basic characteristics of an organism. In an organism, chromosomes are the main carriers of genetic information. The karyotype is the number, size and shape of chromosomes present in medium-term cells of an organism. Genome size refers to the total amount of DNA in one copy of a genome, which is the same as haploid nuclear DNA content (Wolf, Ritter, Atkin, & Ohno, 1969). Varying greatly among species, it is fairly constant within a species. Usually, genome size is estimated by calculating the C-value. The research on karyotype could detect the chromosome mutation and reveal the evolutionary relationship among species (Niu & Cai, 2006). A prior estimation of the genome size is a valuable tool for genomic studies such as whole genome sequencing. China has a rich cavefish fauna. In southwest China, over 10 cavefish species from the genus Triplophysa have been reported (e.g., Li et al., 2008; Romero, Zhao, & Chen, 2009; Zheng, Du, Chen, & Yang, 2009). Cavefishes have evolved constructive and regressive changes in their phenotypes: an increased size and number of specialized mechanosensory organs, eye degeneration and loss of pigmentation (e.g., Jeffery, 2001, 2009). Triplophysa rosa is an endangered cave-dwelling fish found in Wulong County, Chongqing, southwestern China (Chen & Yang, 2005). Thus far, only a very few studies have been carried out on its biology including genetics (Wang et al., 2012; Zhao, Zhao, & Peng, 2014). Many aspects such as cytogenetic and phylogeny are underrepresented. Herein, we studied the karyotype of T. rosa by culturing kidney cells and measured its genome size based on flow cytometry. This is the first report of karyotype and genome size for T. rosa.
Times Cited:
1
Related Records:
Wu, He and Li (2005)
Linemacheilus wulong
Chen, X.Y. and Yang, J. (2005)
Triplophysa rosa sp. nov.: A new blind loach from China
Wu, Q., He, L.J., Zhen, S.M. and Li, L.G. (2007)
White bubble blind fish - Wulong Li rod-shaped loach Heminoemacheilus wulongensis
Wang, J.J., Tang, Q.Y., Wang, Z.J., Zhang, Y.G., Wu, Q. and Peng, Z.G. (2012)
The complete mitogenome sequence of a cave loach Triplophysa rosa (Telestei, Balitoridae, Nemacheilinae)
Kottelat, M. (2012)
Draconectes narinosus, a new genus and species of cave fish from an island of Halong Bay, Vietnam (Teleostei: Nemacheilidae).
Huang, J. (2012)
Comparative studies on the gross anatomy and histological structure of the central nervous system between. In: Triplophysa bleekeri and Triplophysa rosa
Zhao, J., Zhao, K. and Peng, Z. (2014)
Development and characterization of eleven microsatellite markers for an endangered cavefish (Triplophysa rosa Chen and Yang, 2005) using 454 sequencing
Wang, Y., Shen, Y., Feng, C., Zhao, K., Song, Z., Zhang, Y., Yang, L. and He, S. (2016)
Mitogenomic perspectives on the origin of Tibetan loaches and their adaptation to high altitude
Zhang, C.G. and Zhou, Y.H. (2016)
Species diversity and distribution of inland fishes in China
Niu, Y., Zhao, Q., Zhao, H., Ludwig, A. and Peng, Z. (2016)
Karyotype and genome size of an endangered cavefish (Triplophysa rosa Chen & Yang, 2005)
Li, J.X. Wang, Y., Jina H.F.,Li, W.J., Yan, C.C., Yan, P.F., Zhang, X.Y., He, S.P. and Song, Z.B. (2017)
Identification of Triplophysa species from the Qinghai-Tibetan Plateau (QTP) and its adjacent regions through DNA barcodes
Liu, S., Ludwig, A. and Peng, Z. (2017)
Nine novel microsatellites for the cavefish (Triplophysa rosa Chen)
Niu, Y. (2017)
Comparative histological study of visual organs between Triplophysa rosa and Triplophysa bleekeri
Xiao, Y. (2017)
Genetic basis of the albinism in the cavefish Triplophysa rosa
Wu, W.J., He, A.Y., Yang, J.X. and Du, L.N. (2018)
Description of a new species of Triplophysa (Teleostei: Nemacheilidae) from Guizhou Province, China
Zhang, Y., Wang, Z., Zhang, Z., Wu, M.. Chen, J. and Zhao, Y. (2018)
The complete mitochondrial genome of Triplophysa brevibarba with phylogenetic consideration
Shi, C., Yao, M., Lv, X., Zhao, Q., Peng, Z. and Luo, Y. (2018)
Body and organ metabolic rates of a cave fish, Triplophysa rosa: influence of light and ontogenetic variation
Sgouros, K., Page, L.M., Orlofske, S.A. and Jadin, R.C. (2019)
A revised molecular phylogeny reveals polyphyly in Schistura (Teleostei: Cypriniformes: Nemacheilidae)
Yang, X., Liu, H., Ma, Z., Zou, Y., Zou, M., Mao, Y., Li, X., Wang, H., Chen, T.,Wang, W. and Yang, R. (2019)
Chromosome‐level genome assembly of Triplophysa tibetana, a fish adapted to the harsh high‐altitude environment of the Tibetan Plateau
Zhao, Q., Zhang, R., Xiao, Y., Niu, Y., Shao, F., Li, Y. and Peng, Z. (2020)
Comparative transcriptome profiling of the loaches Triplophysa bleekeri and Triplophysa rosa reveals potential mechanisms of eye degeneration
Wang, Y., Xiao, N., Wang, S., Luo, T., Yang, X., Liu, T. and Zhou, J. (2021)
The complete mitochondrial genome of a cavedwelling loach Triplophysa baotianensis (Teleostei:Nemacheilidae)
Chen, S., Sheraliev, B., Shu, L. and Peng, Z. (2021)
Triplophysa wulongensis, a new species of cave-dwelling loach (Teleostei, Nemacheilidae) from Chongqing, Southwest China
Zhao, Q., Shao, F., Li, Y., Yi, S.V. and Peng, Z. (2022)
Novel genome sequence of Chinese cavefish (Triplophysa rosa) reveals pervasive relaxation of natural selection in cavefish genomes
He, Y. and He, D.K. (2023)
X-ray microtomography study of cranium, pectoral fin and girdle, and Weberian Apparatus development in juvenile Triplophysa stewarti (Nemacheilidae)
Zhang, J.H., Long, R., Jing, Y.Y., Zhang, P., Xu, Y., Xiong, W., Zhu, Y.Q. and Luo, Y.P. (2023)
Loss of behavioral stress response in blind cavefish reduces energy expenditure
Xu, Y., Jing, Y., Zhou, J., Long, R., Meng, J. Yang, Y. and Luo, Y. (2023)
Age, growth, and energy storage of the cavefish Triplophysa rosa
Xu, Y., Jing, Y., Zhou, J., Long, R., Meng, J. Yang, Y. and Luo, Y. (2023)
Age, growth, and energy storage of the subterranean fish Triplophysa rosa (Cypriniformes: Nemacheilidae) from Chongqing, China
Ni, L., Jin, L., Zeng, M., Xu, Y., Wang, Y. and Peng, Z. (2024)
The cavefish Triplophysa rosa has a well-developed adaptive immune system: Evidence from histological and comparative genomic analysis