Journal Article
Karyotype and genome size of an endangered cavefish (Triplophysa rosa Chen & Yang, 2005)
Niu, Y., Zhao, Q., Zhao, H., Ludwig, A. and Peng, Z.
Record Number:
3975
Year:
2016
Journal:
Journal of Applied Ichthyology
Pages:
124-126
Volume:
33
Abstract:
The karyotype and genome size are basic characteristics of an organism.
In an organism, chromosomes are the main carriers of
genetic information. The karyotype is the number, size and shape
of chromosomes present in medium-term
cells of an organism.
Genome size refers to the total amount of DNA in one copy of a
genome, which is the same as haploid nuclear DNA content (Wolf,
Ritter, Atkin, & Ohno, 1969). Varying greatly among species, it is
fairly constant within a species. Usually, genome size is estimated
by calculating the C-value.
The research on karyotype could detect
the chromosome mutation and reveal the evolutionary relationship
among species (Niu & Cai, 2006). A prior estimation of the genome
size is a valuable tool for genomic studies such as whole genome
sequencing.
China has a rich cavefish fauna. In southwest China, over 10
cavefish species from the genus Triplophysa have been reported
(e.g., Li et al., 2008; Romero, Zhao, & Chen, 2009; Zheng, Du,
Chen, & Yang, 2009). Cavefishes have evolved constructive and
regressive changes in their phenotypes: an increased size and
number of specialized mechanosensory organs, eye degeneration
and loss of pigmentation (e.g., Jeffery, 2001, 2009). Triplophysa
rosa is an endangered cave-dwelling
fish found in Wulong County,
Chongqing, southwestern China (Chen & Yang, 2005). Thus far,
only a very few studies have been carried out on its biology including
genetics (Wang et al., 2012; Zhao, Zhao, & Peng, 2014). Many
aspects such as cytogenetic and phylogeny are underrepresented.
Herein, we studied the karyotype of T. rosa by culturing kidney
cells and measured its genome size based on flow cytometry. This is
the first report of karyotype and genome size for T. rosa.
Times Cited:
1
Relevent Species:
Related Records:
Wu, He and Li (2005)
Linemacheilus wulong
Chen, X.Y. and Yang, J. (2005)Triplophysa rosa sp. nov.: A new blind loach from China
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Wang, J.J., Tang, Q.Y., Wang, Z.J., Zhang, Y.G., Wu, Q. and Peng, Z.G. (2012)The complete mitogenome sequence of a cave loach Triplophysa rosa (Telestei, Balitoridae, Nemacheilinae)
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Zhao, J., Zhao, K. and Peng, Z. (2014)Development and characterization of eleven microsatellite markers for an endangered cavefish (Triplophysa rosa Chen and Yang, 2005) using 454 sequencing
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Li, J.X. Wang, Y., Jina H.F.,Li, W.J., Yan, C.C., Yan, P.F., Zhang, X.Y., He, S.P. and Song, Z.B. (2017)Identification of Triplophysa species from the Qinghai-Tibetan Plateau (QTP) and its adjacent regions through DNA barcodes
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