Journal Article

Molecular discrimination of Ancistrus lineages (Siluriformes: Loricariidae) using barcode DNA tool

Splendore de Borba, R., Mariotto, S., Centofante, L., Zawadzki C.H. and Parise-Maltempi, P.P.

Record Number:
4337
Year:
2019
Journal:
Mitochondrial DNA Part A
Abstract:
Although several species of Ancistrus have been described from the Amazon and Paraguay river basins in the states of Amazonas and Mato Grosso, Brazil, the taxonomic status of most specimens from these regions remains doubtful. In the present work, cytogenetic and molecular data were used to discriminate and isolate unexpected Ancistrus lineages from the Amazon and Paraguay basins. For that, it was used DNA barcoding based on mitochondrial Cytochrome Oxidase Subunit I (COI) gene and cytogenic data to perform such molecular discrimination. The analyzed sequences had 669 bp, of which 171 bp were conserved and 491 bp were variable. The Neighbor-joining and Bayesian analysis revealed 21 distinct groups in topology. The genetic distances within each group was 0.4%, 21 times smaller than the mean distance observed among groups, which was 8.4%. These values showed seven distinct lineages of Ancistrus from the studied points of the Amazon basin and eight lineages from the Paraguay basin points. Our results illustrate the efficiency of this technique for the discrimination of the Ancistrus lineages once it indicates the occurrence of cryptic species in these regions, which cannot yet be identified either with just chromosomal or morphological analyzes.
Times Cited:
4
Related Records:
Reis, R. E. (1986)
Uma nova especie de Ancystrus de caverna: o primeiro Loricariidae cego (Pisces, Siluriformes)
Reis, R. E. (1987)
Ancistrus cryptophthalmus sp. n., a blind mailed catfish from the Tocantins river basin, Brazil (Pisces, Siluriformes,Loricariidae)
Rodriguez, G. and Bosque, C. (1990)
A stygobiont crab Chaceus caecus n. sp. and its related stygophile species Chaceus motiloni Rodriguez, 1980 (Crustacea, Decapoda, Pseudothelphusidae) from a cave in the Cordillera de Perija, Venezuela
Bleckmann, H., Niemann, U. and Fritzsch, B. (1991)
Peripheral and central aspects of the acoustic and lateral line system of a bottom dwelling catfish, Ancistrus sp.
Bleckmann, H., Niemann, U. and Fritzsch, B. (1991)
Peripheral and central aspects of the acoustic and lateral line system of a bottom dwelling catfish, Ancistrus sp.
Bleckmann, H., Niemann, U. and Fritzsch, B. (1991)
Peripheral and central aspects of the acoustic and lateral line system of a bottom dwelling catfish, Ancistrus sp.
Bleckmann, H., Niemann, U. and Fritzsch, B. (1991)
Peripheral and central aspects of the acoustic and lateral line system of a bottom dwelling catfish, Ancistrus sp.
Viloria, A., Herrera, F. and Galan, C. (1992)
Resultados preliminares del estudio del material biologico colectado en Mesa Turik y Cuenca del Rio Socuy
Trajano, E. and de Souza, A. M. (1994)
Behaviour of Ancistrus cryptophthalmus an armoured blind catfish from caves of central Brazil, with notes on syntopic Trichomycterus sp. (Siluriformes, Loricariidae, Trichomycteridae)
Trajano, E. (1994)
Comparative study of the brain and olfactory organ of the troglobitic catfish Pimelodella kronei (Ribeiro, 1907) and its putative ancestor P. transitoria (Ribeiro, 1912) (Siluriformes, Pimelodidae)
Muller, S., Rapp Py-Daniel, L.H. and Zuanon, J. (1994)
Ancistrus ranunculus, a new species of loricariid fish (Siluriformes: Loricariidae) from the Xingú and Tocantins rivers, Brazil
Perez, A. and Viloria, A. (1994)
Ancistrus galani n. sp. (Siluriforme: Loricariidae), with comments on biospeological explorations in western Venezuela
Sabino, J. and Trajano, E. (1997)
A new species of blind armoured catfish, genus Ancistrus, from caves of Bodoquena region, Mato Grosso do Sul, southwestern Brazil (Siluriformes, Loricariidae, Ancistrinae)
Sabino, J. and Trajano, E. (1997)
A new species of blind armoured catfish, genus Ancistrus, from caves of Bodoquena region, Mato Grosso do Sul, southwestern Brazil (Siluriformes, Loricariidae, Ancistrinae)
Trajano, E. and Gerhard, P. (1997)
Light reaction in Brazilian cave fishes (Siluriformes: Pimelodidae, Trichomycteridae, Loricariidae)
Trajano, E. (2001)
Habitat and population data of troglobitic armored cave catfish, Ancistrus cryptophthalmus Reis, 1987, from central Brazil (Siluriformes: Loricariidae)
Bessa, E. and Trajano, E. (2001)
Light recation and cryptobiotic habitats in armoured catfishes, genus Ancistrus, from caves in central and north-eastern Brazil
Bessa, E. and Trajano, E. (2001)
Light reaction and cryptobiotic habits in two cave-dwelling Brazilian armoured catfishes, genus Ancistrus (Siluriformes: Loricariidae)
Bessa, E. and Trajano, E. (2001)
Light recation and cryptobiotic habitats in armoured catfishes, genus Ancistrus, from caves in central and north-eastern Brazil
DoNascimiento, C., Villarreal, O. and Provenzano, F. (2001)
Descripcion de una nueve especie de bagre anoftalmo del genero Trichomycterus (Siluriformes, Trichomycteridae), de una vueva de la Sierra de Perija, Venezuela
Bessa, E. and Trajano, E. (2001)
Light reaction and cryptobiotic habits in two cave-dwelling Brazilian armoured catfishes, genus Ancistrus (Siluriformes: Loricariidae)
Moller, D. and Parzefall, J. (2001)
Single or multiple origin of the subterranean catfish Ancistrus cryptophthalmus. What we can learn from molecular data
Fernandez, L. and Bichuette, M.E. (2002)
A new cave dwelling species of Ituglanis from the Sao Domingos karst, central Brazil (Siluriformes: Trichomycteridae)
Trajano, E and Reis, RE (2002)
Geometric morphometric study of epigean and cave populations of armoured catfish genus Ancistrus from the Sao Domingos karst area, upper Tocantins basin, central Brazil (Siluriformes: Loricariidae)
Bichuette, M.E. and Trajano, E (2003)
Epigean and subterranean ichthyofauna from the Sao Domingos karst area, Upper Tocantins River basin, central Brazil
Armbruster, J.W. (2004)
Phylogenetic relationships of the suckermouth armoured catfishes (Loricariidae) with emphasis on the Hypostominae and the Ancistrinae
Armbruster, J.W. (2004)
Phylogenetic relationships of the suckermouth armoured catfishes (Loricariidae) with emphasis on the Hypostominae and the Ancistrinae
Armbruster, J.W. (2004)
Phylogenetic relationships of the suckermouth armoured catfishes (Loricariidae) with emphasis on the Hypostominae and the Ancistrinae
Armbruster, J.W. (2004)
Phylogenetic relationships of the suckermouth armoured catfishes (Loricariidae) with emphasis on the Hypostominae and the Ancistrinae
Reis, R.E., Trajano, E. and Hingst-Zaher, E. (2006)
Shape variation in surface and cave populations of the armoured catfish Ancistrus (Siluriformes: Loricariidae) from the São Domingos karst area, Upper Tocantins River, Brazil.
Reis, R.E., Trajano, E. and Hingst-Zaher, E. (2006)
Shape variation in surface and cave populations of the armoured catfish Ancistrus (Siluriformes: Loricariidae) from the São Domingos karst area, Upper Tocantins River, Brazil.
Reis, R.E., Trajano, E. and Hingst-Zaher, E. (2006)
Shape variation in surface and cave populations of the armoured catfish Ancistrus (Siluriformes: Loricariidae) from the São Domingos karst area, Upper Tocantins River, Brazil.
Reis, R.E., Trajano, E. and Hingst-Zaher, E. (2006)
Shape variation in surface and cave populations of the armoured catfish Ancistrus (Siluriformes: Loricariidae) from the São Domingos karst area, Upper Tocantins River, Brazil.
Trajano, E. and Bichuette, M.E. (2007)
Population ecology of cave armoured catfish, Ancistrus cryptophthalmus Reis 1987, from central Brazil (Siluriformes: Loricariidae)
Borghezan, R. (2008)
Caracterizacao da ictiocenose em poc¸oes da Gruta das Fadas e aspectos biologicos de Rhamdia sp. (Siluriformes: Heptapteridae) e Ancistrus sp. (Siluriformes: Loricariidae), Serra da Bodoquena, Mato Grosso do Sul.
Borghezan, R. (2008)
Caracterizacao da ictiocenose em poc¸oes da Gruta das Fadas e aspectos biologicos de Rhamdia sp. (Siluriformes: Heptapteridae) e Ancistrus sp. (Siluriformes: Loricariidae), Serra da Bodoquena, Mato Grosso do Sul.
Cordeiro, L.M (2008)
Fauna cavernıcola da Serra da Bodoquena: revisao bibliografica e um estudo de ecologia de comunidades.
Felice, V., Visconti, M.A. and Trajano, E. (2008)
Mechanisms of pigmentation loss in subterranean fishes
Felice, V., Visconti, M.A. and Trajano, E. (2008)
Mechanisms of pigmentation loss in subterranean fishes
Galán, C., Herrera, F. and Rincón, A. (2009)
Biodiversidad y biomasa global de la Cueva de Los Laureles (Sierra de Perijá, Venezuela)
Secutti, S. and Trajano, E. (2009)
Reproductive behavior, development and eye regression in the cave armored catfish, Ancistrus cryptophthalmus Reis, 1987 (Siluriformes: Loricariidae), breed in laboratory
Trajano, E. and Bichuette, M.E. (2010)
Subterranean fishes of Brazil
Lujan, N.K. and Armbruster, J.W. (2012)
Morphological and functional diversity of the mandible in suckermouth armored catfishes (Siluriformes: Loricariidae)
Lujan, N.K. and Armbruster, J.W. (2012)
Morphological and functional diversity of the mandible in suckermouth armored catfishes (Siluriformes: Loricariidae)
Lujan, N.K. and Armbruster, J.W. (2012)
Morphological and functional diversity of the mandible in suckermouth armored catfishes (Siluriformes: Loricariidae)
Lujan, N.K. and Armbruster, J.W. (2012)
Morphological and functional diversity of the mandible in suckermouth armored catfishes (Siluriformes: Loricariidae)
Gallão, J.E. and Bichuette, M.E. (2012)
A lista de fauna ameaçada de extinção e os entraves para a Inclusão de espécies – o exemplo dos peixes troglóbios Brasileiros
Cordeiro, L.M., Borghezan, R. and Trajano, E. (2013)
Distribuição, riqueza e conservação dos peixes troglóbios da Serra da Bodoquena, MS (Teleostei: Siluriformes). Distribution, richness and conservation of troglobitic fishes from Serra da Bodoquena, MS (Teleostei)
Borghezan. R. (2013)
Ecologia populacional e cornportamento de peixes subterraneos, Rhamdia sp. n. e Ancistrus sp. n. da area carstica da Serra da Bodoquena, Mato Grosso do Sul (Siluriformes: Heptapteridae, Loricariidae)
von Schimonsky, D.M. and Bichuette, M.E. (2013)
A expressão do comportamento agonístico em Ituglanis ramiroi Bichuette and Trajano 2004 (Siluriformes: Trichomycteridae) e sua aplicação para conservação
Cordeiro, L.M., Borghezan, R. and Trajano, E. (2013)
Distribuição, riqueza e conservação dos peixes troglóbios da Serra da Bodoquena, MS (Teleostei: Siluriformes). Distribution, richness and conservation of troglobitic fishes from Serra da Bodoquena, MS (Teleostei)
Borghezan. R. (2013)
Ecologia populacional e cornportamento de peixes subterraneos, Rhamdia sp. n. e Ancistrus sp. n. da area carstica da Serra da Bodoquena, Mato Grosso do Sul (Siluriformes: Heptapteridae, Loricariidae)
von Schimonsky, D.M. and Bichuette, M.E. (2013)
A expressão do comportamento agonístico em Ituglanis ramiroi Bichuette and Trajano 2004 (Siluriformes: Trichomycteridae) e sua aplicação para conservação
Bastos, V.A.A., Ferreira, R.L., de Carvalho, D.C., Pugedo, M.L. and Pinto, L.D.A. (2013)
The cave environment influencing the lipid profile and hepatic lipogenesis of the fish Ancistrus cryptophthalmus Reis, 1987 (Siluriformes: Loricariidae)
Cordeiro, L.M., Borghezan, R. and Trajano, E. (2014)
Subterranean biodiversity in the Serra da Bodoquena karst area, Paraguay river basin, Mato Grosso do Sul, Southwestern Brazil
Cordeiro, L.M., Borghezan, R. and Trajano, E. (2014)
Subterranean biodiversity in the Serra da Bodoquena karst area, Paraguay river basin, Mato Grosso do Sul, Southwestern Brazil
Gallão, J.E. and Bichuette, M.E. (2015)
Taxonomic distinctness and conservation of a new high biodiversity subterranean area in Brazil
Trajano, E., Gallao, J.E. and Bichuette, M.E. (2016)
Spots of high biodiversity of troglobites in Brazil: the challenge of measuring subterranean diversity
Trajano, E., Gallao, J.E. and Bichuette, M.E. (2016)
Spots of high biodiversity of troglobites in Brazil: the challenge of measuring subterranean diversity
Gallão, J.E. and Bichuette M.E. (2018)
Brazilian obligatory subterranean fauna and threats to the hypogean environment
Gallão, J.E. and Bichuette M.E. (2018)
Brazilian obligatory subterranean fauna and threats to the hypogean environment
Bichuette, M.E., Gimenez, E.A., Arnone, I.S. and Trajano, E. (2018)
An important site for conservation of bats in Brazil: Passa Três cave, São Domingos karst area, with an updated checklist for Distrito Federal (DF) and Goiás state
Gallão, J.E. and Bichuette M.E. (2018)
Brazilian obligatory subterranean fauna and threats to the hypogean environment
Bichuette, M.E., Simões, L.B., Zepon, T., von Schimonsky, D.M. and Gallão, J.E. (2019)
Richness and taxonomic distinctness of cave invertebrates from the northeastern state of Goiás, central Brazil: a vulnerable and singular area
Roxo, F.F., Ochoa, L.E., Sabaj, M.H., Lujan, N.K., Covain, R., Silva, G.S.C., Melo, B.F., Albert, J.S., Chang, J., Foresti, F., Alfaro, M.E. and Oliveira, C. (2019)
Phylogenomic reappraisal of the Neotropical catfish family Loricariidae (Teleostei: Siluriformes) using ultraconserved elements
Splendore de Borba, R., Mariotto, S., Centofante, L., Zawadzki C.H. and Parise-Maltempi, P.P. (2019)
Molecular discrimination of Ancistrus lineages (Siluriformes: Loricariidae) using barcode DNA tool
Splendore de Borba, R., Mariotto, S., Centofante, L., Zawadzki C.H. and Parise-Maltempi, P.P. (2019)
Molecular discrimination of Ancistrus lineages (Siluriformes: Loricariidae) using barcode DNA tool
Splendore de Borba, R., Mariotto, S., Centofante, L., Zawadzki C.H. and Parise-Maltempi, P.P. (2019)
Molecular discrimination of Ancistrus lineages (Siluriformes: Loricariidae) using barcode DNA tool
Splendore de Borba, R., Mariotto, S., Centofante, L., Zawadzki C.H. and Parise-Maltempi, P.P. (2019)
Molecular discrimination of Ancistrus lineages (Siluriformes: Loricariidae) using barcode DNA tool
Mendes, I.S., Prosdocimi, F., Schomaker-Bastos, A., Furtado, C., Ferreira, R.L., .Pompeu, P.S. and Carvalho, D.C. (2019)
On the evolutionary origin of Neotropical cavefish Ancistrus cryptophthalmus (Siluriformes, Loricariidae) based on the mitogenome and genetic structure of cave and surface populations
Mendes, I.S., Prosdocimi, F., Schomaker-Bastos, A., Furtado, C., Ferreira, R.L., .Pompeu, P.S. and Carvalho, D.C. (2019)
On the evolutionary origin of Neotropical cavefish Ancistrus cryptophthalmus (Siluriformes, Loricariidae) based on the mitogenome and genetic structure of cave and surface populations
Mendes, I.S., Prosdocimi, F., Schomaker-Bastos, A., Furtado, C., Ferreira, R.L., .Pompeu, P.S. and Carvalho, D.C. (2019)
On the evolutionary origin of Neotropical cavefish Ancistrus cryptophthalmus (Siluriformes, Loricariidae) based on the mitogenome and genetic structure of cave and surface populations
Mendes, I.S., Prosdocimi, F., Schomaker-Bastos, A., Furtado, C., Ferreira, R.L., .Pompeu, P.S. and Carvalho, D.C. (2019)
On the evolutionary origin of Neotropical cavefish Ancistrus cryptophthalmus (Siluriformes, Loricariidae) based on the mitogenome and genetic structure of cave and surface populations
Glugoski, L., Deon, G., Schott, S., Vicari, M.R., Nogaroto, V. and Moreira-Filho, O. (2020)
Comparative cytogenetic analyses in Ancistrus species (Siluriformes: Loricariidae)
Glugoski, L., Deon, G., Schott, S., Vicari, M.R., Nogaroto, V. and Moreira-Filho, O. (2020)
Comparative cytogenetic analyses in Ancistrus species (Siluriformes: Loricariidae)
Glugoski, L., Deon, G., Schott, S., Vicari, M.R., Nogaroto, V. and Moreira-Filho, O. (2020)
Comparative cytogenetic analyses in Ancistrus species (Siluriformes: Loricariidae)
Glugoski, L., Deon, G., Schott, S., Vicari, M.R., Nogaroto, V. and Moreira-Filho, O. (2020)
Comparative cytogenetic analyses in Ancistrus species (Siluriformes: Loricariidae)
Meng, F., Yin, X., Zhang, T., Zhao, C., Xue, X., Xia, X., Zhu, X., Duan, Z., Liu, B. and Liu, Y. (2021)
The first determination and analysis of the complete mitochondrial genome of Ancistrus temmincki (Siluriformes:Loricariidae)
Meng, F., Yin, X., Zhang, T., Zhao, C., Xue, X., Xia, X., Zhu, X., Duan, Z., Liu, B. and Liu, Y. (2021)
The first determination and analysis of the complete mitochondrial genome of Ancistrus temmincki (Siluriformes:Loricariidae)
Meng, F., Yin, X., Zhang, T., Zhao, C., Xue, X., Xia, X., Zhu, X., Duan, Z., Liu, B. and Liu, Y. (2021)
The first determination and analysis of the complete mitochondrial genome of Ancistrus temmincki (Siluriformes:Loricariidae)
Meng, F., Yin, X., Zhang, T., Zhao, C., Xue, X., Xia, X., Zhu, X., Duan, Z., Liu, B. and Liu, Y. (2021)
The first determination and analysis of the complete mitochondrial genome of Ancistrus temmincki (Siluriformes:Loricariidae)
Bichuette, M.E. and Gallão, J.E. (2021)
Under the surface: what we know about the threats to subterranean fishes in Brazil
Santos da Silva, K., Glugoski, L., Vicari, M.R., de Souza, A.C.P., Noronha, R.C.R., Pieczarka, J.C. and Nagamachi, C.Y. (2022)
Chromosomal diversification in Ancistrus species (Siluriformes: Loricariidae) Inferred from repetitive sequence analysis
Santos da Silva, K., Glugoski, L., Vicari, M.R., de Souza, A.C.P., Noronha, R.C.R., Pieczarka, J.C. and Nagamachi, C.Y. (2022)
Chromosomal diversification in Ancistrus species (Siluriformes: Loricariidae) Inferred from repetitive sequence analysis
Santos da Silva, K., Glugoski, L., Vicari, M.R., de Souza, A.C.P., Noronha, R.C.R., Pieczarka, J.C. and Nagamachi, C.Y. (2022)
Chromosomal diversification in Ancistrus species (Siluriformes: Loricariidae) Inferred from repetitive sequence analysis
Santos da Silva, K., Glugoski, L., Vicari, M.R., de Souza, A.C.P., Noronha, R.C.R., Pieczarka, J.C. and Nagamachi, C.Y. (2022)
Chromosomal diversification in Ancistrus species (Siluriformes: Loricariidae) Inferred from repetitive sequence analysis