Journal Article

Lens Defects in Astyanax mexicanus Cavefish: Evolution of Crystallins and a Role for alphaA-Crystallin

Hinaux, Helene, Blin, Maryline, Fumey, Julien, Legendre, Laurent, Heuze, Aurelie, Casane, Didier and Retaux, Sylvie

Record Number:
2902
Year:
2015
Journal:
Developmental Neurobiology
Pages:
505-521
Volume:
75
Short Title:
Lens Defects in Astyanax mexicanus Cavefish: Evolution of Crystallins and a Role for alphaA-Crystallin
Notes:
retaux@inaf.cnrs-gif.fr
URL:
://ZOOREC:ZOOR15110065376
Accession Number:
Zoorec:Zoor15110065376
Language:
English
Issue:
5
Abstract:
The fish Astyanax mexicanus presents, within the same species, populations of river-dwelling surface fish (SF) and blind cave-living fish. In cavefish (CF), the eyes develop almost normally during embryogenesis. But 40 h after fertilization, the lens enters apoptosis, triggering the progressive degeneration of the entire eye. Before apoptosis, the CF lens expresses early differentiation factors correctly. Here, we searched for possible late differentiation defects that would be causal in CF lens degeneration. We reasoned that crystallins, the major lens structural proteins, could be defective or misregulated. We surveyed the CF and SF transcriptomes and uncovered 14 Astyanax crystallins from the beta, gamma, lambda, mu, and zeta families. These proteins are less polymorphic and accumulate more fixed mutations, some at highly conserved positions, in CF than in SF, suggesting relaxed selection at these loci in CF. In situ hybridizations and qPCR show that crybb1c, crybgx, crygm5 are expressed at much lower levels or are not expressed in the CF lens. For the best crystallin candidates, we tested a potential causal role in CF lens apoptosis. Crybgx, crybb1c (not expressed in CF from very early on), and cryaa (previously shown to be faintly expressed in CF) failed to induce any defect when knocked-down in zebrafish embryos. However, the anti-apoptotic cryaa protected lens cells from apoptosis when reexpressed by transgenesis in CF, suggesting a cell-autonomous effect of cryaa on lens cell survival. Altogether, these data suggest that crystallin sequence evolution and expression defects may contribute to the loss of eyes in CF. [copyright] 2014 Wiley Periodicals, Inc. Develop Neurobiol 75: 505-521, 2015
Times Cited:
1
Author Address:
France
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(1964)Boucquey, C., Thines, G. and Van Der Borght, C. (1965)Thines, G., Wolff-Van Ermengem, F., Boucquey, C. and Soffie, M. (1965)Peters, N. and Peters, G. (1966)Reed, M. (1966)Thines, G., Soffie, M. and Vandenbussche, E. (1966)Carmignani, M. P. A. (1966)Cavicchioli, G. and Guarnieri, P. (1966)Sadoglu, P. (1967)Schemmel, C. (1967)Pfeiffer, W. (1967)Pfeiffer, W. (1967)Popper, A. N. and Tavolga, W. N. (1967)Walters, V. and Liu, R. K. (1967)Wilkens, H. (1968)Mattheij, J. A. (1968)Mattheij, J. A. (1968)Carmignani, M. P. A. (1968)Sadoglu, P. and McKee, A. (1969)Mattheij, J. A. (1969)Mattheij, J. A. and Sprangers, J. A. P. (1969)Weiss, B. A. (1969)Whitt, G. S. and Maeda, F. S. (1970)Wilkens, H. (1970)Wilkens, H. (1970)Wilkens, H. (1970)Popper, A. N. (1970)Mattheij, J. A. (1970)Mattheij, J. A. (1970)Gertychowa, R. (1970)Gertychowa, R. (1970)Weiss, B. and Martini, J. (1970)Wiley, S. and Mitchell, R. W. (1971)Wiley, S. and Mitchell, R. W. (1971)Zaccone, G. (1971)Wilkens, H. (1971)Popper, A. N. (1971)Gertychowa, R. (1971)Avise, J. C. and Selander, R. K. (1972)Zaccone, G. (1972)Wilkens, H. (1972)Wilkens, H. (1972)Wilkens, H. (1972)Thines, G. and Wissocq, N. (1972)Wilkens, H. and Burns, R. J. (1972)Schemmel, C. (1973)Zeitlin, S. M. (1973)Zeitlin, S. M. and McDevitt, D. S. (1973)Durand, J. P. (1973)Thines, G. and LeGrain, J. M. (1973)Voneida, T. J. (1973)Mitchell, R. W. (1973)Yasuda, K. (1973)Chakraborty, R. and Nei, M. (1974)Schemmel, C. (1974)Schemmel, C. (1974)Sligar, C. (1974)Sadoglu, P. (1975)Peters, N., Scholl, A. and Wilkens, H. (1975)Omura, Y. (1975)Herwig, H. J. (1976)Herwig, H. J. (1976)Wilkens, H. (1976)Durand, J. P. (1976)Sligar, C. and Voneida, T. J. (1976)Erckens, W. and Weber, F. (1976)Voneida, T. J. and Sligar, C. (1976)Yew, D. T. and Yoshihara, H. M. (1977)Zaccone, G. (1977)Wilkens, H. (1977)Kirby, R. F., Thompson, K. W. and Hubbs, C. (1977)Mitchell, R. W., Russell, W. H. and Elliott, W. R. (1977)Durand, J. P. (1978)Thines, G. and Weyers, M. (1978)Sadoglu, P. (1979)Durand, J. P. (1979)Wilkens, H., Peters, H. and Schemmel, C. (1979)Woodhead, A. D. and Achey, P. M. (1979)Schemmel, C. (1980)Wilkens, H. (1980)Wilkens, H. (1980)Quinn, T. P. (1980)Weissert, R. (1980)Herwig, H. J. (1981)Reddell, J. R. (1981)Campenhausen, C., Riess, I. and Weissert, R. (1981)Weissert, R. and Campenhausen, C. (1981)Tabata, M. (1982)Erckens, W. and Martin, W. (1982)Jankowska, M. and Thines, G. (1982)Erckens, W. and Martin, W. (1982)Lamprecht, G. and Weber, F. (1982)Rose, F. L. and Mitchell, R. W. (1982)Parzefall, J. (1983)Zilles, K., Tillmann, B. and Bennemann, R. (1983)Romero, A. (1983)Wilkens, H. (1984)Romero, A. (1984)Voneida, T. J. and Fish, S. E. (1984)Romero, A. (1984)Schuppa, M. (1984)Teyke, T. (1985)Wilkens, H. (1985)Lamprecht, G. and Weber, F. (1985)Burchards, H., Dolle, A. and Parzefall, J. (1985)Romero, A. (1985)Parzefall, J. (1986)Hassan, E. S. (1986)De Fraipont, M. (1986)Fraipont, M. (1986)De Fraipont, M. and Thines, G. (1986)Huppop, K. (1986)De Fraipont, M. and Thines, G. (1986)Wilkens, H. (1986)Wilkens, H. and Huppop, K. (1986)Romero, A. (1986)De Fraipont, M. (1987)De Fraipont, M. (1987)Huppop, K. (1987)Wilkens, H. (1987)De Fraipont, M. (1988)Huppop, K. (1988)Teyke, T. (1988)Wilkens, H. (1988)Fricke, D. (1988)Hassan, E. S. (1989)Huppop, K. (1989)Teyke, T. (1989)Fack, H. and Wilkens, H. (1989)Langecker, T. G. (1989)Fricke, D. and Parzefall, J. (1989)Abdel-Latif, H., Hassan, E. S. and von Campenhausen, C. (1990)Yokoyama, R. and Yokoyama, S. (1990)Yokoyama, R and Yokoyama, S (1990)Parzefall, J. and Fricke, D. (1990)Peters, N., Schmidt, W. and Fricke, D. (1990)Teyke, T. (1990)Klimpel, B. and Parzefall, J. (1990)Langecker, T. G. (1990)Langecker, T. G., Wilkens, H. and Junge, P. (1991)Parzefall, J. and Fricke, D. (1991)Huppop, K. and Wilkens, H. (1991)Huppop, K. (1991)Riedel, G. and Krug, L. (1991)Campenhausen, C. (1991)Parzefall, J. (1992)Parzefall, J. (1992)Hassan, E. S., Abdel-Latif, H. and Biebricher, R. 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